Dendrigraft Poly-l-lysine: A Non-Immunogenic Synthetic Carrier for Antibody Production - Agropolis Accéder directement au contenu
Article Dans Une Revue Biomacromolecules Année : 2010

Dendrigraft Poly-l-lysine: A Non-Immunogenic Synthetic Carrier for Antibody Production

Résumé

An easily synthesized DendriGraft poly-lysine DGL-G3 (third generation) was shown to act as an efficient carrier for raising antibodies directed against small molecules. The immunological properties of three different forms of DGL-G3 were investigated: the native form (molecular weight 22 kDa bearing a mean number of 123 surface amino groups as TFA salts), a form modified at the C-terminus by fluorescein (fluorescein-DGL-G3), and last a surface-modified form bearing histamine (DGL-G3-Histamine). Our studies demonstrate the native DGL-G3 to be inefficient in eliciting antibody production in rabbits. Immunizations of rabbits using the core-modified fluorescein-DGL-G3 or the surface-modified DGL-G3-histamine conjugate failed in eliciting antibody production. Conversely, following a primary immunization using a BSA-histamine conjugate, a second immunization with DGL-G3-histamine conjugate improved the production of specific hapten-directed antibodies, which demonstrates the utility of DGL-G3 as a carrier for the production of highly specific antibody against haptens.
Fichier non déposé

Dates et versions

hal-00554413 , version 1 (10-01-2011)

Identifiants

Citer

Bernard Romestand, Jean-Luc Rolland, Auguste Commeyras, Gaelle Coussot, Isabelle Desvignes, et al.. Dendrigraft Poly-l-lysine: A Non-Immunogenic Synthetic Carrier for Antibody Production. Biomacromolecules, 2010, 11 (5), pp.1169-1173. ⟨10.1021/bm9012056⟩. ⟨hal-00554413⟩
91 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More