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Article Dans Une Revue Nature Structural and Molecular Biology Année : 2017

Xist-dependent imprinted X inactivation and the early developmental consequences of its failure

Tao Liu
Jun-Bin Liang
  • Fonction : Auteur
Chong-Jian Chen
  • Fonction : Auteur

Résumé

The long noncoding RNA Xist is expressed from only the paternal X chromosome in mouse preimplantation female embryos and mediates transcriptional silencing of that chromosome. In females, absence of Xist leads to postimplantation lethality. Here, through single-cell RNA sequencing of early preimplantation mouse embryos, we found that the initiation of imprinted X-chromosome inactivation absolutely requires Xist. Lack of paternal Xist leads to genome-wide transcriptional misregulation in the early blastocyst and to failure to activate the extraembryonic pathway that is essential for postimplantation development. We also demonstrate that the expression dynamics of X-linked genes depends on the strain and parent of origin as well as on the location along the X chromosome, particularly at the first 'entry' sites of Xist. This study demonstrates that dosage-compensation failure has an effect as early as the blastocyst stage and reveals genetic and epigenetic contributions to orchestrating transcriptional silencing of the X chromosome during early embryogenesis.
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Dates et versions

hal-03021809 , version 1 (19-11-2021)

Identifiants

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Maud Borensztein, Laurène Syx, Katia Ancelin, Patricia Diabangouaya, Christel Picard, et al.. Xist-dependent imprinted X inactivation and the early developmental consequences of its failure. Nature Structural and Molecular Biology, 2017, ⟨10.1038/nsmb.3365⟩. ⟨hal-03021809⟩
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