Cell clusters adopt a collective amoeboid mode of migration in confined nonadhesive environments - Institut Curie Access content directly
Journal Articles Science Advances Year : 2022

Cell clusters adopt a collective amoeboid mode of migration in confined nonadhesive environments

Emmanuel Dornier
Emilie Gontran
Ananyo Maitra
Jérôme Cartry
Marceline Lebel
Fanny Jaulin

Abstract

Cell migration is essential to living organisms and deregulated in cancer. Single cell’s migration ranges from traction-dependent mesenchymal motility to contractility-driven propulsive amoeboid locomotion, but collective cell migration has only been described as a focal adhesion–dependent and traction-dependent process. Here, we show that cancer cell clusters, from patients and cell lines, migrate without focal adhesions when confined into nonadhesive microfabricated channels. Clusters coordinate and behave like giant super cells, mobilizing their actomyosin contractility at the rear to power their migration. This polarized cortex does not sustain persistent retrograde flows, of cells or actin, like in the other modes of migration but rather harnesses fluctuating cell deformations, or jiggling. Theoretical physical modeling shows this is sufficient to create a gradient of friction forces and trigger directed cluster motion. This collective amoeboid mode of migration could foster metastatic spread by enabling cells to cross a wide spectrum of environments.
Fichier principal
Vignette du fichier
Cell clusters adopt a collective amoeboid mode of migration in confined nonadhesive environments .pdf (9 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03808168 , version 1 (20-10-2022)

Identifiers

Cite

Diane-Laure Pagès, Emmanuel Dornier, Jean de Seze, Emilie Gontran, Ananyo Maitra, et al.. Cell clusters adopt a collective amoeboid mode of migration in confined nonadhesive environments. Science Advances , 2022, 8 (39), eabp8416. ⟨10.1126/sciadv.abp8416⟩. ⟨hal-03808168⟩
60 View
41 Download

Altmetric

Share

Gmail Facebook X LinkedIn More