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Chapitre D'ouvrage Année : 2018

LiveFly: Live imaging of mRNA transcription in Drosophila Embryos

Carmina Angelica Perez-Romero
  • Fonction : Auteur
Huy Tran
  • Fonction : Auteur
Mathieu Coppey
Aleksandra Walczak
  • Fonction : Auteur
Cécile Fradin
  • Fonction : Auteur

Résumé

Live imaging has been used in recent years for the understanding of dynamic processes in biology, such as embryo development. This was made possible by a combination of advancements in microscopy, leading to improved signal-to-noise ratios and better spatial and temporal resolutions, and by the development of new fluorescence markers, allowing for the quantification of protein expression and transcriptional dynamics in vivo. Here we describe a general protocol, which can be used in standard confocal microscopes to image early Drosophila melanogaster embryos, in order to learn about the transcriptional dynamics of a fluorescently labelled RNA.
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Dates et versions

hal-04100580 , version 1 (17-05-2023)

Identifiants

Citer

Carmina Angelica Perez-Romero, Huy Tran, Mathieu Coppey, Aleksandra Walczak, Cécile Fradin, et al.. LiveFly: Live imaging of mRNA transcription in Drosophila Embryos. Morphogen Gradients, 1863, pp.183-195, 2018, Methods in Molecular Biology, ⟨10.1007/978-1-4939-8772-6_10⟩. ⟨hal-04100580⟩
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