Naturally occurring steroids in Xenopus oocyte during meiotic maturation. Unexpected presence and role of steroid sulfates - Biologie de l'ovocyte Accéder directement au contenu
Article Dans Une Revue Molecular and Cellular Endocrinology Année : 2012

Naturally occurring steroids in Xenopus oocyte during meiotic maturation. Unexpected presence and role of steroid sulfates

Olivier Haccard
Philippe Liere
  • Fonction : Auteur
Antoine Pianos
  • Fonction : Auteur
Bernard Eychenne
  • Fonction : Auteur
Catherine Jessus
  • Fonction : Auteur
Rene Ozon
  • Fonction : Auteur

Résumé

In the ovary, oocytes are surrounded by follicle cells and arrested in prophase of meiosis I. Although steroidogenic activity of follicle cells is involved in oogenesis regulation, clear qualitative and quantitative data about the steroid content of follicles are missing. We measured steroid levels of Xenopus oocytes and follicles by gas chromatography-mass spectrometry. We show that dehydroepiandrosterone sulfate is the main steroid present in oocytes. Lower levels of free steroids are also detected, e.g., androgens, whereas progesterone is almost undetectable. We propose that sulfatation is a protective mechanism against local variations of active steroids that could be deleterious for follicle-enclosed oocytes. Steroid levels were measured after LH stimulation, responsible for the release by follicle cells of a steroid signal triggering oocyte meiosis resumption. Oocyte levels of androgens rise slowly during meiosis re-entry whereas progesterone increases abruptly to micromolar concentration, therefore representing the main physiological mediator of meiosis resumption in Xeno pus oocyte.
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Dates et versions

hal-01538514 , version 1 (13-06-2017)

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Citer

Olivier Haccard, Aude Dupre, Philippe Liere, Antoine Pianos, Bernard Eychenne, et al.. Naturally occurring steroids in Xenopus oocyte during meiotic maturation. Unexpected presence and role of steroid sulfates. Molecular and Cellular Endocrinology, 2012, 362 (1-2), pp.110-119. ⟨10.1016/j.mce.2012.05.019⟩. ⟨hal-01538514⟩
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