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Article Dans Une Revue Journal of visualized experiments : JoVE Année : 2021

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Natasja Costermans
  • Fonction : Auteur
Enrico Daldello
  • Fonction : Auteur
Ria Marathe
  • Fonction : Auteur
Marco Conti
  • Fonction : Auteur

Résumé

Abstract Oocytes are held in meiotic prophase for prolonged periods until hormonal signals trigger meiotic divisions. Key players of M-phase entry are the opposing Cdk1 kinase and PP2A-B55δ phosphatase. In Xenopus , the protein Arpp19, phosphorylated at serine 67 by Greatwall, plays an essential role in inhibiting PP2A-B55δ, promoting Cdk1 activation. Furthermore, Arpp19 has an earlier role in maintaining the prophase arrest through a second serine (S109) phosphorylated by PKA. Prophase release, induced by progesterone, relies on Arpp19 dephosphorylation at S109, owing to an unknown phosphatase. Here, we identified this phosphatase as PP2A-B55δ. In prophase, PKA and PP2A-B55δ are simultaneously active, suggesting the presence of other important targets for both enzymes. The drop in PKA activity induced by progesterone enables PP2A-B55δ to dephosphorylate S109, unlocking the prophase block. Hence, PP2A-B55δ acts critically on Arpp19 on two distinct sites, opposing PKA and Greatwall to orchestrate the prophase release and M-phase entry.
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Dates et versions

hal-03989230 , version 1 (14-02-2023)

Identifiants

Citer

Natasja Costermans, Enrico Daldello, Ria Marathe, Marco Conti. Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay. Journal of visualized experiments : JoVE, 2021, 12 (172), pp.1837. ⟨10.3791/62041⟩. ⟨hal-03989230⟩
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