Biotin sulfone as a new tool for synthetic oligosaccharide immobilization: application to multiple analysis profiling and surface plasmonic analysis of anti-Candida albicans antibody reactivity against alpha and beta (1-->2) oligomannosides. - Université Pierre et Marie Curie Accéder directement au contenu
Article Dans Une Revue Journal of Medicinal Chemistry Année : 2008

Biotin sulfone as a new tool for synthetic oligosaccharide immobilization: application to multiple analysis profiling and surface plasmonic analysis of anti-Candida albicans antibody reactivity against alpha and beta (1-->2) oligomannosides.

Résumé

As a part of our glycoantigen synthetic program for diagnosis and basic analysis of yeast-related pathogenic mechanisms, a library of 1-->2 oligomannosides suitable for immunoanalysis was prepared. The use of biotin sulfone, an oxidized form of biotin, offers a convenient solution for both oligosaccharide synthesis and immobilization on microspheres and surface plasmon resonance sensors. The application of this new strategy for the analysis of anti- Candida albicans antibody response through multiple-analyte profiling technology (Luminex) and with surface plasmonic analysis using biotin tagged synthetic oligosaccharides on avidin coated surfaces was validated using monoclonal antibodies.

Domaines

Chimie organique

Dates et versions

hal-00813494 , version 1 (15-04-2013)

Identifiants

Citer

Mayeul Collot, Boualem Sendid, Aurélie Fievez, Camille Savaux, Annie Standaert-Vitse, et al.. Biotin sulfone as a new tool for synthetic oligosaccharide immobilization: application to multiple analysis profiling and surface plasmonic analysis of anti-Candida albicans antibody reactivity against alpha and beta (1-->2) oligomannosides.. Journal of Medicinal Chemistry, 2008, 51 ((19)), pp.6201-10. ⟨10.1021/jm800099g⟩. ⟨hal-00813494⟩
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