Castanospermine: a potent inhibitor of sucrase from the human enterocyte-like cell line Caco-2. - Université Pierre et Marie Curie Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 1986

Castanospermine: a potent inhibitor of sucrase from the human enterocyte-like cell line Caco-2.

Résumé

The addition of castanospermine (5-50 microM) to a culture medium of Caco-2 cells results in a specific suppression of sucrase activity without modification of the biosynthesis of the enzyme. This effect is due to a direct inhibiting effect of castanospermine on Caco-2 sucrase activity. This inhibition is time-dependent (half-maximum efficiency at 10 min for 100 nM), enhanced by preincubation (suggesting a strong interaction with the enzyme), dose-dependent (ED50 at 4 nM after 1 h preincubation period) and of the fully non-competitive type. The calculated Ki (2.6 nM) suggests that castanospermine is the most potent inhibitor of sucrase so far reported.

Domaines

Chimie organique
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Dates et versions

hal-00814099 , version 1 (16-04-2013)

Identifiants

  • HAL Id : hal-00814099 , version 1
  • PUBMED : 2867936

Citer

G. Trugnan, Monique Rousset, A. Zweibaum. Castanospermine: a potent inhibitor of sucrase from the human enterocyte-like cell line Caco-2.. FEBS Letters, 1986, 195 ((1-2)), pp.28-32. ⟨hal-00814099⟩
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