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Article Dans Une Revue The Plant cell Année : 2016

Diatom Phytochromes Reveal the Existence of Far-Red-Light-Based Sensing in the Ocean

Gen Enomoto
  • Fonction : Auteur
Raffaella Raniello
  • Fonction : Auteur
Marie J. J. Huysman
  • Fonction : Auteur
Chris Bowler
Masahiko Ikeuchi
  • Fonction : Auteur

Résumé

The absorption of visible light in aquatic environments has led to the common assumption that aquatic organisms sense and adapt to penetrative blue/green light wavelengths but show little or no response to the more attenuated red/far-red wavelengths. Here, we show that two marine diatom species, Phaeodactylum tricornutum and Thalassiosira pseudonana, possess a bona fide red/far-red light sensing phytochrome (DPH) that uses biliverdin as a chromophore and displays accentuated red-shifted absorbance peaks compared with other characterized plant and algal phytochromes. Exposure to both red and far-red light causes changes in gene expression in P. tricornutum, and the responses to far-red light disappear in DPH knockout cells, demonstrating that P. tricornutum DPH mediates far-red light signaling. The identification of DPH genes in diverse diatom species widely distributed along the water column further emphasizes the ecological significance of far-red light sensing, raising questions about the sources of far-red light. Our analyses indicate that, although far-red wavelengths from sunlight are only detectable at the ocean surface, chlorophyll fluorescence and Raman scattering can generate red/far-red photons in deeper layers. This study opens up novel perspectives on phytochrome-mediated far-red light signaling in the ocean and on the light sensing and adaptive capabilities of marine phototrophs.

Dates et versions

hal-01528502 , version 1 (29-05-2017)

Identifiants

Citer

Antonio Emidio Fortunato, Marianne Jaubert, Gen Enomoto, Jean-Pierre Bouly, Raffaella Raniello, et al.. Diatom Phytochromes Reveal the Existence of Far-Red-Light-Based Sensing in the Ocean. The Plant cell, 2016, 28 (3), pp.616-628. ⟨10.1105/tpc.15.00928⟩. ⟨hal-01528502⟩
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