RAL-1 controls multivesicular body biogenesis and exosome secretion - Université Pierre et Marie Curie Access content directly
Journal Articles Journal of Cell Biology Year : 2015

RAL-1 controls multivesicular body biogenesis and exosome secretion

Vincent Hyenne
  • Function : Author
Ahmet Apaydin
  • Function : Author
David Rodriguez
  • Function : Author
Coralie Spiegelhalter
  • Function : Author
Sarah Hoff-Yoessle
  • Function : Author
Maxime Diem
  • Function : Author
Saurabh Tak
  • Function : Author
Olivier Lefebvre
  • Function : Author
Yannick Schwab
Jacky G. Goetz
  • Function : Author


Exosomes are secreted vesicles arising from the fusion of multivesicular bodies (MVBs) with the plasma membrane. Despite their importance in various processes, the molecular mechanisms controlling their formation and release remain unclear. Using nematodes and mammary tumor cells, we show that Ral GTPases are involved in exosome biogenesis. In Caenorhabditis elegans, RAL-1 localizes at the surface of secretory MVBs. A quantitative electron microscopy analysis of RAL-1-deficient animals revealed that RAL-1 is involved in both MVB formation and their fusion with the plasma membrane. These functions do not involve the exocyst complex, a common Ral guanosine triphosphatase (GTPase) effector. Furthermore, we show that the target membrane SNARE protein SYX-5 colocalizes with a constitutively active form of RAL-1 at the plasma membrane, and MVBs accumulate under the plasma membrane when SYX-5 is absent. In mammals, RalA and RalB are both required for the secretion of exosome-like vesicles in cultured cells. Therefore, Ral GTPases represent new regulators of MVB formation and exosome release.

Dates and versions

hal-01538541 , version 1 (13-06-2017)



Vincent Hyenne, Ahmet Apaydin, David Rodriguez, Coralie Spiegelhalter, Sarah Hoff-Yoessle, et al.. RAL-1 controls multivesicular body biogenesis and exosome secretion. Journal of Cell Biology, 2015, 211 (1), pp.27-37. ⟨10.1083/jcb.201504136⟩. ⟨hal-01538541⟩
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