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Journal Articles Annual Review of Biophysics and Biomolecular Structure Year : 2011

Amphipols From A to Z

Christine Ebel
Frank Gabel
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Yann Y. Gohon
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Erik Goormaghtigh
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Frank Wien
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Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.
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hal-02327349 , version 1 (07-12-2022)





Jean-Luc Popot, Thorsten Althoff, Dominique Bagnard, Jean-Louis Banères, Paola Bazzacco, et al.. Amphipols From A to Z. Annual Review of Biophysics and Biomolecular Structure, 2011, 40 (1), pp.379-408. ⟨10.1146/annurev-biophys-042910-155219⟩. ⟨hal-02327349⟩
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