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Communication Dans Un Congrès Année : 2013

Biological control of microbial communities producing biohydrogen

Résumé

A wide number of microbial species issued from different environments are able to produce biohydrogen by dark fermentation. More particularly, microbial mixed cultures are interesting since they present a broad metabolic flexibility and allow considering complex organic biomass as potential resources for hydrogen production. However, microbial diversity leads to process instability because of the presence of multiple bacterial metabolisms that can lead to direct H2 uptake. To date, only few controllers, essentially physicochemical, are available to control bioprocesses producing bioH2. In this study, biotic control of microbial ecosystems was investigated through the identification of keystone species within metabolic networks and their use as biological trigger of the whole microbial fermentative metabolism, in order to improve stability and conversion efficiencies of organic substrates to biohydrogen. Innovative advanced molecular tools were first developed to monitor microbial populations and specific functional genes (hydA) associated with hydrogen production. These tools led to the identification of "keystone" species in natural ecosystems (MEE -- Microbial Ecosystem Engineer). Their addition de novo in new ecosystems improved the hydrogen yields nearly 2.5 times of the nominal value, and stabilized substantially the bioprocess. A fine study of how a MEE strain can interact with H2-producing bacteria showed a new type of non-trophic interaction by direct and mandatory contact between these microorganisms, exchanging cellular constituents. Beyond this study, it is expected that these findings lead to new biotechnological or environmental applications through the use of biotic control of microbial metabolisms.
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Dates et versions

hal-02809567 , version 1 (06-06-2020)

Identifiants

  • HAL Id : hal-02809567 , version 1
  • PRODINRA : 221518

Citer

Eric Trably, Jérôme J. Hamelin, Eric Latrille, Yan Rafrafi, Isabelle Meynial-Salles, et al.. Biological control of microbial communities producing biohydrogen. BioH2 2013, Aug 2013, Montreal, Canada. ⟨hal-02809567⟩
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