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Article Dans Une Revue Nucleic Acids Research Année : 2014

Targeting vertebrate intron-encoded box C/D 2′-O-methylation guide RNAs into the Cajal body

Résumé

Post-transcriptional pseudouridylation and 2-Omethylation of splicesomal small nuclear ribonucleic acids (snRNAs) is mediated by box H/ACA and box C/D small Cajal body (CB)-specific ribonucleoproteins (scaRNPs), respectively. The WD-repeat protein 79 (WDR79) has been proposed to interact with both classes of modification scaRNPs and target them into the CB. The box H/ACA scaRNAs carry the common CAB box motif (consensus, ugAG) that is required for both WDR79 binding and CB-specific accumulation. Thus far, no cis-acting CB-localization element has been reported for vertebrate box C/D scaR-NAs. In this study, systematic mutational analysis of the human U90 and another newly identified box C/D scaRNA, mgU2-47, demonstrated that the CBspecific accumulation of vertebrate intron-encoded box C/D scaRNAs relies on GU-or UG-dominated dinucleotide repeat sequences which are predicted to form the terminal stem-loop of the RNA apical hairpin. While the loop nucleotides are unimportant, the adjacent terminal helix that is composed mostly of consecutive G.U and U.G wobble base-pairs is essential for CB-specific localization of box C/D scaRNAs. Co-immunoprecipitation experiments confirmed that the newly identified CB localization element, called the G.U/U.G wobble stem, is crucial for in vivo association of box C/D scaRNPs with WDR79.
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Dates et versions

hal-04118801 , version 1 (06-06-2023)

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Aline Marnef, Patrica Richard, Natalia Pinzón, Tamás Kiss. Targeting vertebrate intron-encoded box C/D 2′-O-methylation guide RNAs into the Cajal body. Nucleic Acids Research, 2014, 42 (10), pp.6616-6629. ⟨10.1093/nar/gku287⟩. ⟨hal-04118801⟩
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