Structure, Orientation and Stability of Lysozyme Confined in layered Materials - Université Pierre et Marie Curie Access content directly
Journal Articles The Royal Society of Chemistry Year : 2013

Structure, Orientation and Stability of Lysozyme Confined in layered Materials

Abstract

The confinement of lysozyme in 3 layered materials based on montmorillonite and lamellar double hydroxide exhibiting different surface charges was studied. The protein structure and orientation in these materials were determined by X-Ray diffraction, time resolved fluorescence and fluorescence anisotropy. For both Montmorillonite exchanged with sodium and modified with non-ionic surfactant (tri-ethylene glycol mono n-decyl ether), the lysozyme was found to be located in the interlayer space with "end-on" and "side-on" orientation, respectively. Conversely no lysozyme intercalation was observed with lamellar double hydroxide modified with anionic surfactant (sodium octylsulfate), since protein was adsorbed on the surface of the particles. Fourier transformed infra-red spectroscopy analysis shows that lysozyme confinement in the interlayer space preserves its structure after dehydration, whereas some structural changes were observed for lysozyme adsorbed on the particle surface.
Fichier principal
Vignette du fichier
manuscript-revised.pdf (1012.19 Ko) Télécharger le fichier
Vignette du fichier
GA.gif (21.03 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Format : Figure, Image
Loading...

Dates and versions

insu-00779421 , version 1 (22-01-2013)
insu-00779421 , version 2 (20-02-2013)

Identifiers

Cite

Sébastien Balme, Régis Guégan, Jean-Marc Janot, Maguy Jaber, Mathilde Lepoitevin, et al.. Structure, Orientation and Stability of Lysozyme Confined in layered Materials. The Royal Society of Chemistry, 2013, 9 (11), pp.3188-3196. ⟨10.1039/C3SM27880H⟩. ⟨insu-00779421v2⟩
694 View
546 Download

Altmetric

Share

Gmail Facebook X LinkedIn More